FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244261
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT21440.8777496202832216No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT5430.22230319207732713RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT4240.17358481296645803RNA PCR Primer, Index 29 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4550.071.84347570
TATACAC3700.050.3762973
CTTATAC3650.050.1647571
TTATACA3800.049.0506062
AAGGGGG4800.046.74820769
CTCGTAT4300.041.59621439
TCGTATG4450.041.12365340
TCTCGTA4350.041.01397738
ATAAGAC350.001120883240.9208851
GTATCAA8450.040.5797541
TACACAT4850.039.1176765
CGTATGC4600.039.11511241
GCCGTCT4650.038.64952546
CCGTCTT4700.038.3130947
GGTATCA3400.038.1869131
GCTAATC4700.038.16625633
CGTCTTC4750.038.07516548
AATCTCG4700.037.76054436
TGCCGTC4950.037.55188445
CTAATCT4800.037.4803534
Produced by FastQC (version 0.11.5)