FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180948
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29600.039.9955941
GGTATCA12700.037.8276251
TATCAAC33900.034.8197062
TCAACGC35100.033.725624
ATCAACG35400.033.4383933
CAACGCA35600.033.1555635
CTCGTAT1154.5474735E-1133.11055439
AACGCAG35900.032.9712836
ACGCAGA38600.030.7538857
AGAGTAC40800.029.09558911
TCGTATG1202.564775E-928.87345340
CGCAGAG41300.028.8264148
CAGAGTA42800.027.81732210
GCAGAGT43950.027.0883059
GACGGAC1653.6379788E-1227.05558626
AGTACGG32350.026.72621513
GAGTACG33550.025.77028512
GTGGTAT5100.025.5680851
TGGTATC5900.024.4266342
GTACGGG35850.024.0212614
Produced by FastQC (version 0.11.5)