FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180948
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15440.13074242049607604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29700.039.8573841
GGTATCA12500.038.9784431
TATCAAC34100.034.6674352
TCAACGC34100.034.5493854
ATCAACG34300.034.349393
CAACGCA34550.034.0002675
AACGCAG35350.033.133936
ACGCAGA37300.031.3099127
TAAGTCG450.004183454431.29886458
CGCAGAG38050.030.6027578
AGAGTAC39900.029.10292211
CAGAGTA40850.028.58897410
GCAGAGT42000.027.5615529
CGACCTA1306.2318577E-926.5685536
AGTACGG35550.026.1130713
GAGTACG36300.025.57354212
TACGGGG15300.024.6342815
GTACGGG38300.023.79091314
ACGGGGC5500.023.67634816
CATAGCG1350.002045377422.89584770
Produced by FastQC (version 0.11.5)