FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548963
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGCCTGGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTT10470.19072323635654861No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8570.15611252488783395No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8130.14809741275823687No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCGATAAGTGCCACTGCTGTCTG8120.14791525111892787No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC8070.14700444292238274No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCCGATAAGTG7790.14190391702173005No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC7510.13680339112107737No Hit
CTATTACTGTCAGTCAGCAGACAGCAGTGGCACTTATCGGAGGGTGTTCG7450.13571042128522323No Hit
GTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC7220.13152070358111567No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGG7200.1311563803024976No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCAC6840.12459856128737275No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG6800.12386991473013664No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG6610.12040884358326517No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6460.11767641899362981No Hit
CAGTAATAGTCACCCTCGTCCTCTGCCCGGACTCCACTGATGGTCAACGT6420.1169477724363937No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6380.11621912587915761No Hit
GGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGGGAATGCATCTCCAGAGCAGGTGATCCTGGCCGT6280.11439750948606738No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT6220.11330453965021321No Hit
GGCCAGGCCAGGCCCCTATACTATTGATATATGAGGACACTGAGAGGCCC6220.11330453965021321No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6210.1131223780109042No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC5850.10656455899577932No Hit
CCCCTATACTATTGATATATGAGGACACTGAGAGGCCCTCAGGGATCCCT5720.104196457684762No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG5680.1034678111275259No Hit
CCTATGAGCTGACACAGCCGCCCTCGGTGTCAGTGTCCCCGGGACAGACGGCCAGGATCACCTGCTCTGGAGATG5610.10219267965236273No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG5510.10037106325927249No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC5500.10018890161996347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4500.043.4411
TCGTATG557.4061372E-637.6223740
ACTAGAT557.603905E-637.47817232
AAGTCCG700.001807135337.1346770
GTGGTAT1500.036.5818981
GTATCAA10000.035.6673471
CTCGTAT601.3497502E-534.4682339
ACCGTAC601.3935149E-534.3080327
TATACAC701.0193853E-634.2924043
TATCAAC11000.032.4248622
TTATACA751.746319E-632.009162
TCTCGTA652.350078E-531.78481538
CGTACTA652.4049936E-531.6776129
ATCAACG11850.030.0962873
TCAACGC11850.030.0962874
TGGTATC1850.029.6609952
GTACTAG703.989227E-529.41492730
CAACGCA12150.029.3531675
AACGCAG12250.029.113556
CTAGATC756.317232E-527.4965433
Produced by FastQC (version 0.11.5)