FastQCFastQC Report
Wed 8 May 2024
EGAF00005761119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288000
Total Bases96 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19470.15116459627329193No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19370.150388198757764No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC18850.14635093167701863No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17480.1357142857142857No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17280.1341614906832298No Hit
GTATAATCACTGGCAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA16140.1253105590062112No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15040.11677018633540373No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT14890.11560559006211181No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14820.11506211180124225No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14630.11358695652173914No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13750.10675465838509317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)