FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822400
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTACTCATTTGCAGAAACA23660.28769455252918286No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22220.2701848249027237No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20820.25316147859922183No Hit
GTTAGTAGCCGCTTGGCCTGGTATCAGCAGAGACCAGGCAAAGCCCCTAA19950.24258268482490272No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18650.2267752918287938No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17990.21875000000000003No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17740.2157101167315175No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17450.21218385214007782No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17040.20719844357976655No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16630.20221303501945526No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16630.20221303501945526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15970.19418774319066148No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15930.19370136186770429No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15040.1828793774319066No Hit
GTATAATAATTTCCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT14580.1772859922178988No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13860.16853112840466924No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13320.1619649805447471No Hit
CCTTCCACCCTGTCTGCTTCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACACTGTTAGTAGCCG12890.1567363813229572No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGCAACTATGCCATGACATGGGTCCGCCAGGCTCCAGGGAAGGGG12700.1544260700389105No Hit
TTATTATACTGTTGGCAGTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC12380.1505350194552529No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12330.14992704280155641No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12290.14944066147859922No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12120.14737354085603113No Hit
GTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCCCTTGGCCGAAGGTC12040.14640077821011674No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11880.14445525291828792No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11750.14287451361867703No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11720.14250972762645914No Hit
GTTTATTACTGTGCGACATCCAGAGAGAATTACGATATTTGGAGACATCC11680.14202334630350194No Hit
GTATGTGCCACCACCACTACCACTAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCATGT11660.14178015564202334No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT11650.14165856031128404No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11470.13946984435797666No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11250.13679474708171205No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11100.13497081712062256No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTG11060.13448443579766536No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10880.13229571984435798No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10590.12876945525291827No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10550.12828307392996108No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10510.1277966926070039No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTGCAACTTATTACTGCCAACAGTATAATAATTTC10420.1267023346303502No Hit
GGTATCAGCAGAGACCAGGCAAAGCCCCTAACCTCCTGATCTCTGAGACGTCTACTTTAGAAAGTGGGGTCTCAT10410.12658073929961092No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA10390.1263375486381323No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCATGTCATGGCATAGTTGC10380.126215953307393No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10290.1251215953307393No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10150.1234192607003891No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10090.1226896887159533No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10020.12183852140077822No Hit
GTGTGAGGGGCAGCTCTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC9970.1212305447470817No Hit
GGCACATACTACGCAGACTCCGTGAAGGGCCGGCTCACCATCTCCAGAGACAATTCCGGGAACACGCTGTTTCTG9790.11904182879377433No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9730.11831225680933853No Hit
GTCTGCTTCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACA9690.11782587548638133No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGCTCACCATCTCCAGAGACAATT9590.11660992217898832No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9590.11660992217898832No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9380.11405642023346303No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG9380.11405642023346303No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA9360.11381322957198442No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9170.11150291828793775No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA9160.11138132295719844No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9140.11113813229571984No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9100.11065175097276264No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9070.11028696498054476No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9010.10955739299610895No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC8960.10894941634241245No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8930.10858463035019456No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG8910.10834143968871596No Hit
CTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATCTGGTGAT8740.10627431906614787No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT8730.10615272373540857No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA8710.10590953307392996No Hit
GTCTCCTTCCACCCTGTCTGCTTCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACACTGTTAGTAG8590.10445038910505836No Hit
ACCTTGATGAGACCCCACTTTCTAAAGTAGACGTCTCAGAGATCAGGAGGTTAGGGGCTTTGCCTGGTCTCTG8570.10420719844357977No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8540.10384241245136186No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8540.10384241245136186No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGGGCAGCTCTTGGAGT8540.10384241245136186No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8410.10226167315175098No Hit
GTATTAGTGGTAGTGGTGGTGGCACATACTACGCAGACTCCGTGAAGGGCCGGCTCACCATCTCCAGAGACAATT8400.10214007782101168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG707.2759576E-1249.42653740
ATGCCGT751.6370905E-1146.19917744
CGTATGC751.6370905E-1146.15963741
GTGGTAT1300.042.3783261
CTTATAC856.730261E-1140.5086941
TATACAC856.730261E-1140.5086943
GTATCAA13750.039.5659451
CTCGTAT901.200533E-1038.4170439
TATGCCG1003.6561687E-1034.63666543
ACGAGAC1003.8744474E-1034.44076522
TATCAAC16050.034.3251232
ATCAACG16350.033.69533
TCAACGC16550.033.2860874
CAACGCA16600.032.9784165
CATCTCG1056.366463E-1032.88272536
AACGCAG16850.032.489126
GTATGCC1101.0168151E-931.48017942
TAATTTG553.289204E-431.30978623
CGAGACG1101.0768417E-931.30978623
GCAGCGC5500.031.3021743
Produced by FastQC (version 0.11.5)