FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822400
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22250.2705496108949416No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21370.2598492217898833No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTACTCATTTGCAGAAACA20820.25316147859922183No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18290.222397859922179No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17150.20853599221789884No Hit
GTTAGTAGCCGCTTGGCCTGGTATCAGCAGAGACCAGGCAAAGCCCCTAA16850.20488813229571987No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16380.19917315175097278No Hit
TTATTATACTGTTGGCAGTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC15670.1905398832684825No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15580.1894455252918288No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15500.1884727626459144No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15290.1859192607003891No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14110.17157101167315175No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14060.17096303501945526No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13560.16488326848249027No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13550.16476167315175097No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13510.16427529182879377No Hit
GTATAATAATTTCCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT12970.1577091439688716No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12870.1564931906614786No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12720.1546692607003891No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12680.15418287937743191No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGCAACTATGCCATGACATGGGTCCGCCAGGCTCCAGGGAAGGGG12310.14968385214007782No Hit
GTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCCCTTGGCCGAAGGTC11760.14299610894941633No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11530.14019941634241245No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11360.13813229571984434No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11350.13801070038910507No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT11300.13740272373540857No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11210.13630836575875485No Hit
GTTTATTACTGTGCGACATCCAGAGAGAATTACGATATTTGGAGACATCC11020.1339980544747082No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10770.1309581712062257No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10720.1303501945525292No Hit
CCTTCCACCCTGTCTGCTTCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACACTGTTAGTAGCCG10710.1302285992217899No Hit
GTATGTGCCACCACCACTACCACTAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCATGT10580.12864785992217898No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10500.12767509727626458No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10460.1271887159533074No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10390.1263375486381323No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT10360.1259727626459144No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10220.1242704280155642No Hit
GGGCGGGATGTCTCCAAATATCGTAATTCTCTCTGGATGTCGCACAGTAA10150.1234192607003891No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10140.1232976653696498No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTG9970.1212305447470817No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA9800.11916342412451363No Hit
GGTATCAGCAGAGACCAGGCAAAGCCCCTAACCTCCTGATCTCTGAGACGTCTACTTTAGAAAGTGGGGTCTCAT9700.11794747081712062No Hit
GTGTGAGGGGCAGCTCTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC9700.11794747081712062No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC9630.11709630350194554No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9590.11660992217898832No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTGCAACTTATTACTGCCAACAGTATAATAATTTC9560.11624513618677043No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9480.11527237354085602No Hit
GGCACATACTACGCAGACTCCGTGAAGGGCCGGCTCACCATCTCCAGAGACAATTCCGGGAACACGCTGTTTCTG9420.11454280155642023No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGGGCAGCTCTTGGAGT9380.11405642023346303No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9370.11393482490272373No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9240.11235408560311284No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9120.11089494163424125No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCATGTCATGGCATAGTTGC9070.11028696498054476No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9010.10955739299610895No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8960.10894941634241245No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8920.10846303501945524No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8880.10797665369649806No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8810.10712548638132295No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8770.10663910505836575No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGCTCACCATCTCCAGAGACAATT8770.10663910505836575No Hit
GTATTAGTGGTAGTGGTGGTGGCACATACTACGCAGACTCCGTGAAGGGCCGGCTCACCATCTCCAGAGACAATT8680.10554474708171208No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA8630.10493677042801558No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA8630.10493677042801558No Hit
GTCTGCTTCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACA8600.10457198443579767No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8450.10274805447470818No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA8440.10262645914396887No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC8420.10238326848249027No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8400.10214007782101168No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG8400.10214007782101168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG208.679781E-568.729754
GTGGTAT2050.053.7178651
GTATATC350.001364421839.329151
GTATCAA14250.038.880661
TATCAAC16050.034.694482
ATATACC400.002646343534.3648764
ATCAACG16600.033.5327263
TCAACGC16800.033.3421134
TCTGGAG10050.032.87037370
CAACGCA17500.032.0084275
AACGCAG17550.031.9172346
CTCGGAC450.00471431830.542843
AGAGTAC18500.030.27823811
CGCAGAG18500.030.27648
CAGAGTA18800.029.79145410
GCAGAGT19100.029.3235269
CGAACTA500.00781091727.54382529
AGTACGG18200.025.3016113
GAGTACG18700.025.17640712
TTCGGGG9550.024.70809470
Produced by FastQC (version 0.11.5)