FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868131
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22560.25986861429899405No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18680.21517489871920253No Hit
GTTTTTATACTGCTGACAGTAATAGACTGCAAAATCTTCAGACTGCAGGCTACTGATGGTGAGAGTGAACTCCGT15380.17716220247865816No Hit
GAGTATTAGGAGCGACGTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGCTGCATC15020.1730153628887806No Hit
GTATAAAAACTGGCCTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGG14860.17117232307105726No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14780.17025080316219557No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14650.16875333331029535No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13820.1591925642558554No Hit
CAGTAATAGACTGCAAAATCTTCAGACTGCAGGCTACTGATGGTGAGAGTGAACTCCGTCCCAGACCCACTGCCA12660.145830525577361No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12440.1432963458279914No Hit
CTATTACTGTCAGCAGTATAAAAACTGGCCTCCCATGTACACTTTTGGCC12410.14295077586216826No Hit
GTCTATTACTGTCAGCAGTATAAAAACTGGCCTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA12280.14145330601026804No Hit
GTAATAGACTGCAAAATCTTCAGACTGCAGGCTACTGATGGTGAGAGTGAACTCCGTCCCAGACCCACTGCCA11760.1354634266026671No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACGGAGTTCACT11640.13408114673937457No Hit
GAGTTCACTCTCACCATCAGTAGCCTGCAGTCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTATAAAAACTGG11640.13408114673937457No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11130.12820645732038138No Hit
GTCTCCAGCTACCCTGTATTTGTCTCCAGGAGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGGAG11040.12716974742291198No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10760.1239444277418961No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10670.12290771784442671No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10630.12244695788999588No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGAGGCCAGTTTTTATA10510.12106467802670334No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10500.12094948803809563No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGGAGCGACGTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT10420.12002796812923394No Hit
GTATTAGGAGCGACGTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGCTGCATCCA10270.11830011830011829No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10170.1171482184140412No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9290.10701149941656271No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9260.10666592945073959No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8970.10332541978111598No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8920.10274946983807744No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT8830.10171275994060804No Hit
GAGTGAACTCCGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA8780.1011368099975695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)