FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358228
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39300.040.997391
GGTATCA16600.039.856591
TATCAAC45300.035.7120482
CAACGCA46300.034.7862745
TCAACGC46250.034.7497834
ATCAACG47300.033.9783823
AACGCAG48600.033.2092976
ACGCAGA50550.031.9282237
CGCAGAG51650.031.1155558
AGAGTAC52700.030.69067211
CAGAGTA53300.030.34518410
GCAGAGT55000.029.469559
GAGTACG42200.029.39484212
AGTACGG42350.029.20981613
TCGTATG2450.026.85511440
GTACGGG45700.026.6187214
TGGTATC6400.026.2414052
TACGGGA14200.026.0621615
TACGGGG14900.025.29772215
GTGGTAT6300.025.0304241
Produced by FastQC (version 0.11.5)