FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240579
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT252.6253538E-454.9324517
GTATCAA6500.038.566371
GGTATCA2650.037.5795331
GTGGTAT853.085006E-936.3599131
CAACGCA7400.034.803975
TATCAAC7550.034.1125032
TCAACGC7750.033.6752744
ACGAAAA500.002976439233.5380769
ATCAACG7850.033.246293
AACGCAG7850.032.8020176
CGGGAAC553.3433424E-431.2116186
TTACACA902.0666448E-730.524374
ACGCAGA8500.030.2936297
CGCAGAG8500.030.2936298
CAGAGTA8500.030.29362910
AGAGTAC8550.030.11647411
CCATGCG605.4249045E-428.73608435
GTATATG605.558857E-428.6165981
GCAGAGT9250.027.8373879
GTACATA500.007900231527.4719331
Produced by FastQC (version 0.11.5)