FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086782
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTACTCATTTGCAGAAACA26510.24393116558794678No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24990.2299449199563482No Hit
GTTAGTAGCCGCTTGGCCTGGTATCAGCAGAGACCAGGCAAAGCCCCTAA22360.20574503442272693No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22190.20418078326656128No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21360.19654355703351728No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21280.19580743884238055No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20300.1867899910009551No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19830.1824652966280266No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19640.18071701592407677No Hit
TTATTATACTGTTGGCAGTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC19090.17565620336001148No Hit
GTATAATAATTTCCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT18690.17197561240432763No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18650.17160755330875926No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18570.17087143511762248No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17560.16157794295452077No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17520.1612098838589524No Hit
CCTTCCACCCTGTCTGCTTCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACACTGTTAGTAGCCG16240.14943199280076408No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15830.14565938707118814No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15630.14381909159334624No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15570.14326700294999367No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15240.14023051541155448No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15200.1398624563159861No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14890.13700999832533112No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14730.13553776194305756No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14680.1350776880735971No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTG14240.13102903802234486No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT13860.12753247661444522No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13380.12311576746762459No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGCAACTATGCCATGACATGGGTCCGCCAGGCTCCAGGGAAGGGG13370.1230237526937325No Hit
GTTTATTACTGTGCGACATCCAGAGAGAATTACGATATTTGGAGACATCC13320.12256367882427202No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13260.12201159018091945No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13230.12173554585924316No Hit
GTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCCCTTGGCCGAAGGTC13100.12053935379864592No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13070.12026330947696963No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12740.11722682193853046No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG12720.11704279239074626No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12720.11704279239074626No Hit
ACCTTGATGAGACCCCACTTTCTAAAGTAGACGTCTCAGAGATCAGGAGGTTAGGGGCTTTGCCTGGTCTCTG12710.11695077761685416No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12660.11649070374739369No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTGCAACTTATTACTGCCAACAGTATAATAATTTC12340.11354623098284661No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12200.11225802414835727No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12110.11142989118332838No Hit
GTATGTGCCACCACCACTACCACTAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCATGT12090.11124586163554419No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11880.10931355138381019No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCATGTCATGGCATAGTTGC11820.1087614627404576No Hit
GCTACTAACAGTGTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGAAGCAGACAGGGTGGAAGGAGA11730.10793332977542874No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11700.10765728545375246No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11650.10719721158429198No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11570.1064610933931552No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC11410.10498885701088166No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11390.10480482746309748No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11390.10480482746309748No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11240.10342460585471604No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11140.10250445811579506No Hit
GGTATCAGCAGAGACCAGGCAAAGCCCCTAACCTCCTGATCTCTGAGACGTCTACTTTAGAAAGTGGGGTCTCAT11120.1023204285680109No Hit
GTCTGCTTCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACA11080.10195236947244249No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11050.1016763251507662No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11040.10158431037687413No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11030.10149229560298201No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10930.10057214786406105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)