FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086782
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25650.2360178950332265No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTACTCATTTGCAGAAACA24810.22828865402629048No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22340.2055610048749427No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20650.19001050808717848No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19970.18375350346251595No Hit
GTTAGTAGCCGCTTGGCCTGGTATCAGCAGAGACCAGGCAAAGCCCCTAA19900.18310940004527126No Hit
TTATTATACTGTTGGCAGTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC19530.17970485341126372No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19460.17906074999401905No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19450.17896873522012696No Hit
GTATAATAATTTCCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT18440.16967524305702525No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18030.1659026373274493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17640.16231406114565755No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15740.1448312541061593No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15720.1446472245583751No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15650.14400312114113042No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15510.14271491430664107No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15470.14234685521107268No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15320.14096663360269124No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14930.1373780574208995No Hit
CCTTCCACCCTGTCTGCTTCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCACACTGTTAGTAGCCG14300.13158112666569743No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13900.1279005357100136No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13850.12744046184055313No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13800.12698038797109265No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13800.12698038797109265No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13550.12468001862379025No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT13380.12311576746762459No Hit
GTGCAGCCACAGTTCGTTGGATCTCCACCTTGGTCCCTTGGCCGAAGGTC13360.12293173791984041No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13230.12173554585924316No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTAGCAACTATGCCATGACATGGGTCCGCCAGGCTCCAGGGAAGGGG13190.12136748676367477No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12990.11952719128583286No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12930.11897510264248029No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12820.11796294012966722No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTGCAACTTATTACTGCCAACAGTATAATAATTTC12670.11658271852128578No Hit
GTTTATTACTGTGCGACATCCAGAGAGAATTACGATATTTGGAGACATCC12480.11483443781733595No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATCTTG12370.1138222753045229No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12300.11317817188727822No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12120.1115219059572205No Hit
GTATGTGCCACCACCACTACCACTAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCATGT11990.11032571389662324No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11760.10820937409710503No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11660.10728922635818408No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCATGTCATGGCATAGTTGC11600.10673713771483148No Hit
GCTACTAACAGTGTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGAAGCAGACAGGGTGGAAGGAGA11420.10508087178477377No Hit
GTGTGAGGGGCAGCTCTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC11340.104344753593637No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11320.1041607240458528No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11200.10305654675914763No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11200.10305654675914763No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11100.1021363990202267No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGAGACCCCA11040.10158431037687413No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10870.10002005922070847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2250.047.289631
GTATCAA20400.044.2499051
TATCAAC23500.038.6638152
ATCAACG23700.038.330483
TCAACGC24000.037.7033044
CAACGCA24150.037.6110535
AACGCAG24750.036.8360676
AGAGTAC26300.034.6683111
CGCAGAG26400.034.405578
CAGAGTA26550.034.21118510
GCAGAGT27050.033.4521069
ATAATAC450.004769194430.4715293
AGTACGG26600.029.25438513
GAGTACG26650.029.07086812
TTCGGGG10800.028.61452370
CGACTAT500.00706848528.11402758
GACAGGT2450.028.0188731
TCTGGAG11200.027.59257770
TAACGGC756.208542E-527.57029536
TTTAACG756.249745E-527.54353134
Produced by FastQC (version 0.11.5)