FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434371
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9790.22538337043679252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGCG200.006915726752.56380554
GTATCAA14300.040.2058141
GGTATCA6100.037.0279541
TCAACGC15400.036.4198844
ATCAACG15550.036.284323
CAACGCA15600.035.9529655
AACGCAG16250.034.72536
TATCAAC16450.034.507032
ACGCAGA16800.033.5884637
AGAGTAC17650.031.77712411
CAGAGTA17550.031.7633210
CGCAGAG17650.031.583368
GTATAAA553.4113097E-431.1116431
AGTAGCC450.004618788630.66764835
AGTACGG15050.030.45325913
GAGTACG15300.030.17920512
GCAGAGT18800.029.8333119
GTGGTAT1900.028.8192061
TACGGGG7150.028.22678815
GTACGGG16700.027.444414
Produced by FastQC (version 0.11.5)