FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779646
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26830.3441305412969476No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA24160.3098842295092901No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20580.2639659537790228No Hit
GTACTCACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA18430.23638933567285667No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18320.2349784389325412No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17260.22138252488950114No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16470.21124972102723544No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16350.2097105609468913No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15980.2049648173658301No Hit
CGTATATAGTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAG15880.20368218396554333No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC15880.20368218396554333No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTCATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA15030.19277980006310558No Hit
GGCCAAAAGTGTACGGCCAGTGAGTACCTTGCATGCAGTAATAAACCCCA14930.19149716666281877No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14750.18918842654230253No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG14610.18739273978190102No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14350.18405789294115535No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13580.174181615758947No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGGA13580.174181615758947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13540.1736685623988323No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13190.1691793454978285No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12990.16661407869725492No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12620.16186833511619375No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12230.15686606485507526No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATGAGGTTTCTAACCGGGAC12160.1559682214748745No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT11980.15365948135435825No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC11880.15237684795407147No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11690.14993984449352657No Hit
CTATATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC11650.14942679113341184No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11410.14634847097272352No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCT11220.1439114675121786No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11200.14365494083212124No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAAAACTCAGGTTCAGGGAGA11170.1432701508120352No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10950.14044835733140426No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA10870.1394222506111748No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10620.13621566711045782No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10450.13403519032997027No Hit
GTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATGAGGTTT10430.13377866364991292No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10410.13352213696985554No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT10360.13288082026971215No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10150.13018729012910987No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC9990.128135076688651No Hit
GTGAGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC9980.12800681334862232No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9660.12390238646770457No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA9610.12326106976756118No Hit
GGTCTAGTCAAAGCCTCGTATATAGTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC9240.11851532618650004No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9210.11813053616641399No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8980.11518047934575436No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8790.11274347588520946No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT8720.11184563250500867No Hit
AGTGAGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC8400.10774120562409092No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8350.10709988892394753No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8310.10658683556383282No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8300.10645857222380413No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8260.10594551886368943No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8240.10568899218363206No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8170.1047911488034313No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG7980.1023541453428864No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7960.10209761866282903No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT7910.10145630196268564No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTCTCCATCACTATAT7820.1003019319024275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1900.045.2162321
GGTATCA5650.043.791721
TCGTATG758.58563E-1041.4632740
TGGTATC1950.040.529692
GTATCAA13400.039.4933431
CGTATGC852.899469E-936.61593641
TATACAC853.086825E-936.3810963
AGAGCAT5950.035.0723770
TCTATTG4150.034.61208768
CTATTGA4500.034.38430469
GTTCGCT701.0045442E-634.359939
GACGTGT4150.033.19267762
TTGGACG4250.032.25854559
GTGTCTA4200.032.14091565
ACGTGTC4300.032.0855663
CGTGTCT4450.031.8515864
TATCAAC16650.031.782392
TAGTTCG652.3860077E-531.7168567
GTCTATT4400.031.21817267
TCAACGC17100.031.1449644
Produced by FastQC (version 0.11.5)