FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779646
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26370.33823042765562833No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA19410.24895914299566724No Hit
GTACTCACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA18070.2317718554318242No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17770.2279239552309638No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16620.21317367112766564No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16150.20714529414631772No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15900.20393871064560068No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA15730.20175823386511313No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15580.19983428376468293No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCCG15380.19726901696410934No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTCATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA15300.1962429102438799No Hit
GGCCAAAAGTGTACGGCCAGTGAGTACCTTGCATGCAGTAATAAACCCCA14790.18970147990241726No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC14170.1817491528206391No Hit
CGTATATAGTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAG14110.18097957278046703No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12690.16276617849639452No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12630.16199659845622244No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12460.1598161216757349No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12460.1598161216757349No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGGA12360.15853348827544808No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12290.15763564489524734No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11940.15314642799424355No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATGAGGTTTCTAACCGGGAC11590.14865721109323976No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC11560.14827242107315372No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11190.14352667749209258No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11120.1426288341118918No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11070.1419875174117484No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCT11040.14160272739166238No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11030.1414744640516337No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10750.13788309053083064No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10690.1371135104906586No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT10570.1355743504103144No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10570.1355743504103144No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10540.1351895603902284No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10340.1326242935896548No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10010.12839160336870836No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG9900.1269807066283929No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAAAACTCAGGTTCAGGGAGA9900.1269807066283929No Hit
GTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATGAGGTTT9830.12608286324819212No Hit
GTGAGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC9790.12556980988807742No Hit
CTATATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC9770.12531328320802004No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC9520.12210669970730305No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9470.12146538300715966No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA9450.1212088563271023No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9150.11736095612624191No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9120.11697616610615587No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACTCACTGGCCGTACACTTTTGGCCAGGGGA8840.11338479258535285No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8760.1123586858651234No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT8740.11210215918506605No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8650.11094778912480792No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8640.11081952578477926No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8330.10684336224389018No Hit
GCCTCCACCCTGCTGATTTCCAGTGTGAAATCAGTGCCTGACCCACTGCC8230.10556072884360337No Hit
GGTCTAGTCAAAGCCTCGTATATAGTGATGGAGACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC8130.10427809544331658No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8120.10414983210328789No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8020.1028671987030011No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7970.1022258820028577No Hit
AGTGAGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC7950.10196935532280034No Hit
TTATTACTGCATGCAAGGTACTCACTGGCCGTACACTTTTGGCCAGGGGA7830.1004301952424562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCG200.007526288351.4551111
GGTATCA6050.043.6981281
GTATCAA14900.041.938471
GTGGTAT2000.037.7676661
TGGTATC2150.036.6943132
TATCAAC17750.035.170812
ATCAACG17850.034.969293
TCAACGC18250.034.397394
CAACGCA18750.033.4801255
AACGCAG19050.032.592746
CGCAGAG19700.031.3432148
TATTCTA1053.397862E-431.1320870
CAGAGTA20150.030.81150410
AGAGTAC20200.030.73721111
GCAGAGT20600.030.1384379
ACGCAGA21000.029.5662677
TACGGGT1900.028.89264115
AGTACGG21000.027.60607313
AGTGTCG500.00795208927.4427245
GAGTACG21000.027.27937312
Produced by FastQC (version 0.11.5)