FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834395
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34730.4162297233324745No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29800.3571449972734736No Hit
CAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA27920.3346137021434692No Hit
CTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGA27200.32598469549793563No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27200.32598469549793563No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG27070.32442668040915873No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24950.29901904973064314No Hit
GTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA24580.29458469909335505No Hit
GGTCTAGTCAGAGCCTCCAGCATTCTAATGGAAACAACTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTC23360.27996332672175644No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21730.26042821445478465No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21420.25671294770462433No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21400.2564732530755817No Hit
GTCTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20320.24352974310728132No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20090.24077325487329143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20050.24029386561520621No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19090.22878852342116143No Hit
GTCAGGGACCCCGGCGGCCCGCTTAGAACCCAAACAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA18440.22099844797727697No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18110.21704348659807404No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18110.21704348659807404No Hit
CTCTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA17920.21476638762216935No Hit
GTCTCCACTCTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCT17910.21464654030764804No Hit
GACTAGACCTGCAGGTGAGGGAGGCCGGCTCTCCAGCGGTGACGGGCAGG17670.21177020475913688No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGA17570.21057173161392384No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17510.20985264772679607No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA17420.20877402189610436No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17350.20793509069445526No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17220.20637707560567836No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17190.20601753366211445No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17060.20445951857333755No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16530.19810761090370865No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16430.1969091377584957No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16300.19535112266971877No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACT16180.19391295489546317No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16080.1927144817502502No Hit
GCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCG15910.19067707740338807No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15750.1887595203710473No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC14720.1764152469753534No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14590.1748572318865765No Hit
GTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT14470.17341906411232091No Hit
GATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAAT14210.1703030339347671No Hit
CTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG14040.168265629587905No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCC13980.1675465457007772No Hit
GTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGTTCAGTGGCAGT13970.1674266983862559No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG13840.16586868329747903No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13620.16323204237801042No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13590.16287250043444654No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13300.15939692831332883No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13300.15939692831332883No Hit
GCCCTGGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCATTAGAATGC13180.15795876053907323No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13150.15759921859550932No Hit
GTCCAAATAGTTGTTTCCATTAGAATGCTGGAGGCTCTGACTAGACCTGC12880.15436334110343422No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAACT12730.15256563138561471No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12250.14681296028859234No Hit
TCCGAGGAGTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCA12240.14669311297407103No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12090.14489540325625153No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG12020.14405647205460245No Hit
CTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGC12020.14405647205460245No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11670.13986181604635695No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11660.13974196873183564No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11610.13914273215922915No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11520.13806410632853744No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11160.13374960300577063No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGA11160.13374960300577063No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11040.13231143523151506No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10970.13147250402986593No Hit
CTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCTGCAGGTCTA10800.12943509968300385No Hit
GAGTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT10720.12847632116683344No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTC10560.12655876413449266No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10330.12380227590050276No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCA10300.12344273395693886No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10120.12128548229555546No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10110.12116563498103416No Hit
GTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCC10090.12092594035199157No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGTT10020.12008700915034246No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9840.11792975748895908No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9630.11541296388401177No Hit
GTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAAC9570.11469387999688398No Hit
GAGTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGA9570.11469387999688398No Hit
CTCCAGCGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC9560.11457403268236267No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9550.11445418536784138No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9510.11397479610975618No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9500.11385494879523486No Hit
CTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCT9490.11373510148071357No Hit
CTGCAGGTACCAGTCCAAATAGTTGTTTCCATTAGAATGCTGGAGGCTCTGACTAGACCTGCAGGTGAGGGAGGC9460.11337555953714966No Hit
GCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCATTAGAATGCTGGAGGCTCTGA9440.11313586490810706No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9380.11241678102097927No Hit
GCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGT9370.11229693370645798No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9260.11097861324672369No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9180.11001983473055328No Hit
GGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCATTAGAATGCTGGAG9100.10906105621438288No Hit
CCGCTGGAGAGCCGGCCTCCCTCACCTGCAGGTCTAGTCAGAGCCTCCAG9090.10894120889986157No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9090.10894120889986157No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9060.10858166695629767No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGAGTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCAGCCT9010.10798243038369118No Hit
CCACTCTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCTGCAG9000.10786258306916988No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9000.10786258306916988No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8920.10690380455299947No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT8860.10618472066587167No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8820.10570533140778647No Hit
CCCTCACCTGCAGGTCTAGTCAGAGCCTCCAGCATTCTAATGGAAACAACTATTTGGACTGGTACCTGCAGAAGC8750.10486640020613738No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCTGCAGGT8480.10163052271406228No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8410.1007915915124132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT451.9345462E-645.74092539
GGTATCA6850.044.9621431
TCGTATG503.93387E-641.2570640
GTATCAA16850.040.373121
GTGGTAT1650.038.9731831
CGTATGC557.4780855E-637.57275841
TATCAAC19350.034.6322252
CGCAGAT800.002714475234.18317470
TTAACGG400.002741934334.1170935
CTTATAC701.1305074E-633.845131
ATCAACG20250.033.0930143
GCGTATT1500.032.87748351
ATAGCCG1550.032.751398
TCAACGC20600.032.5307544
CAACGCA20700.032.21015
GTCGGTT550.00367669632.13326669
AACGCAG20900.031.9037826
CGTAGTA450.003830416131.86648858
AGCGTAT1550.031.74760650
TAGCCGT1600.031.727919
Produced by FastQC (version 0.11.5)