FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834395
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33790.4049640757674723No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG28130.3371304957484165No Hit
CAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA27400.32838164178836166No Hit
CTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGA25770.30884652952138975No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25710.308127445634262No Hit
GTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA25030.29997782824681357No Hit
GGTCTAGTCAGAGCCTCCAGCATTCTAATGGAAACAACTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTC24290.29110912697223734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23420.28068241060888427No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22580.2706152361890951No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21400.2564732530755817No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20720.24832363568813332No Hit
GTCTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19960.2392152397845145No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC19080.22866867610664013No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18860.22603203518717155No Hit
GTCTCCACTCTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCT18420.22075875334823433No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17960.21524577688025454No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17920.21476638762216935No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17730.21248928864626465No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17520.20997249504131738No Hit
CTCTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA17470.20937325846871085No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17000.20374043468620978No Hit
GTCAGGGACCCCGGCGGCCCGCTTAGAACCCAAACAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA16790.20122364108126245No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16620.1991862367344004No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16420.19678929044397436No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG16410.19666944312945306No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16330.19571066461328265No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16070.19259463443572888No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16070.19259463443572888No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA15440.185044253620887No Hit
GACTAGACCTGCAGGTGAGGGAGGCCGGCTCTCCAGCGGTGACGGGCAGG15380.1843251697337592No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGA15090.1808495976126415No Hit
GCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCG14970.1794114298383859No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACT14940.17905188789482201No Hit
GTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGTTCAGTGGCAGT14840.177853414749609No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14830.1777335674350877No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14550.1743778426284913No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC14490.1736587587413635No Hit
GTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT14440.173059522168757No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14440.173059522168757No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14120.1692244081040754No Hit
GATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAAT13970.1674266983862559No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG13910.1667076144991281No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13830.1657488359829577No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13770.1650297520958299No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG13350.15999616488593532No Hit
CTACAAACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG13180.15795876053907323No Hit
CTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC12960.15532211961960463No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12830.15376410453082773No Hit
GCCCTGGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCATTAGAATGC12820.15364425721630642No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCC12770.15304502064369993No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12580.15076792166779523No Hit
CTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCTGCAGGTCTA12470.14944960120806094No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAACT12350.14801143343380535No Hit
GTCCAAATAGTTGTTTCCATTAGAATGCTGGAGGCTCTGACTAGACCTGC12220.14645341834502842No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12070.14465570862720892No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11980.14357708279651724No Hit
TCCGAGGAGTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCA11960.14333738816747463No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11860.14213891502226164No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11840.14189922039321903No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11600.13902288484470784No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11490.13770456438497353No Hit
CTATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGC11480.13758471707045225No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11070.13267097717507895No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11030.13219158791699376No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10720.12847632116683344No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10450.12524044367475837No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTC10310.12356258127146015No Hit
GTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAAC10160.12176487155364066No Hit
GAGTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT10060.12056639840842766No Hit
CTGCAGGTACCAGTCCAAATAGTTGTTTCCATTAGAATGCTGGAGGCTCTGACTAGACCTGCAGGTGAGGGAGGC9910.11876868869060817No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9900.11864884137608687No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCA9810.11757021554539517No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9790.11733052091635256No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9750.11685113165826735No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9730.11661143702922476No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9670.11589235314209696No Hit
GGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCATTAGAATGCTGGAG9620.11529311656949047No Hit
GAGTGGAGGCTGAGGATGTTGGAGTCTATTACTGCATGCAAACTCTACAAACTCCTCGGACGTTCGGCCAAGGGA9540.11433433805332006No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGA9470.11349540685167098No Hit
CTCCAGCGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC9390.11253662833550057No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9330.11181754444837277No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9320.11169769713385148No Hit
CCGCTGGAGAGCCGGCCTCCCTCACCTGCAGGTCTAGTCAGAGCCTCCAG9280.11121830787576628No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCTGCAGGT9210.11037937667411717No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9160.10978014010151067No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGAGTTTGTAGAGTTTGCATGCAGTAATAGACTCCAACATCCTCAGCCT9120.10930075084342547No Hit
GCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGT9010.10798243038369118No Hit
CCACTCTCCCTGCCCGTCACCGCTGGAGAGCCGGCCTCCCTCACCTGCAG9010.10798243038369118No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8850.10606487335135038No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTGTTTGGGTTCTAAGCGGGCCGCCGGGGTCCCTGACAGGTT8790.10534578946422259No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8760.10498624752065869No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8690.10414731631900957No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8690.10414731631900957No Hit
GCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAGTCCAAATAGTTGTTTCCATTAGAATGCTGGAGGCTCTGA8660.10378777437544569No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8590.10294884317379657No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8450.1012709807704984No Hit
GGGTCTCTGGCTCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCGCTGGAGAGCCGG8430.10103128614145579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)