FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117070
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAACTTCCAGGAAGAGC26080.23346791159014205No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGAGTTGAGCTTCAGGGAGA24090.21565345054472862No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG22660.20285210416536117No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22410.20061410654659065No Hit
GTTTATTACTGTGCGAGATTACCGAAACCAGTGGCTGTCGGGCACTTCGA22050.19739138997556105No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC19390.1735790953118426No Hit
GGTTATGATGTACACTGGTACCAGCAACTTCCAGGAAGAGCCCCCAAACTCCTCATCTTTGATAGTAGCAATCGG18720.16758126169353757No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18370.1644480650272588No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18020.16131486836098008No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCCGAATCA17730.15871879112320625No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17540.15701791293294065No Hit
GTCCTATGACAGTAGCCTGAGTGATTCGGATTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCC17450.15621223379018323No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16720.14967728074337328No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16270.14564888502958634No Hit
GTCTCTGGTGGCTCCATCAGAAGTTATAGTTACTATTGGGCCTGGATCCG16240.14538032531533385No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTGCTA16170.1447536859820781No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16170.1447536859820781No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAAGAGCCCCCAAACTCCTCAT16080.1439480068393207No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA15860.14197856893480265No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15550.1392034518875272No Hit
GGCTACTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGG15180.1358912154117468No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15110.13526457607849104No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14860.13302657845972052No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14710.1316837798884582No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT14560.13034098131719588No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14390.12881914293643193No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14330.128282023507927No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14320.12819250360317616No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG13210.118255794175835No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13010.11646539608081857No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12930.115749236842812No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC12890.1153911572238087No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG12870.11521211741430708No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT12850.11503307760480544No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12800.11458547808105132No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12740.1140483586525464No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC12590.11270556008128407No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12340.11046756246251353No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12320.1102885226530119No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12300.11010948284351026No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12290.11001996293875944No Hit
ACAGTAGCCTGAGTGATTCGGATTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTG11920.10670772646297903No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG11870.10626012693922494No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11780.10545444779646754No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11670.10446972884420852No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11610.10393260941570358No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11470.10267933074919208No Hit
GATTATTACTGCCAGTCCTATGACAGTAGCCTGAGTGATTCGGATTGGGT11420.10223173122543797No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11400.10205269141593633No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11380.1018736516064347No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11270.10088893265417566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGCG200.007506897751.49016618
GGTATCA8200.045.216951
TGGTATC2550.043.0805932
AGGGGGG2350.042.36120670
GTGGTAT2850.040.956741
TACGGAG7550.040.43484570
GTATCAA18300.038.646261
TCTCGTA2000.036.19542738
CGTATGC1850.035.48486341
TCGTATG1850.035.4656940
TATACAC1850.035.2561043
TTATACA1900.034.329852
TATCAAC21400.033.046492
AGCGTCA1208.185452E-1131.4676173
ATCTCGT2300.031.45307537
CGAGACA2350.030.68460723
ATCAACG23200.030.4811733
TCAACGC23600.030.1086544
CAACGCA24050.029.545295
AACGCAG24300.029.238716
Produced by FastQC (version 0.11.5)