FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123078
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18640.1659724435880678No Hit
GTAATACACAGCCGTGTCCTCAGGTCGCAGGCTGTTCATTTGCAGATGCA14420.12839713715343012No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11960.10649304856830959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.038.7759861
GTATCAA26700.037.03121
TATCAAC29700.033.1338042
ATCAACG32450.030.5234033
TCAACGC32700.030.295444
CAACGCA32900.030.1112755
AACGCAG33850.029.4646576
AGAGTAC35050.028.07629211
CGCAGAG36150.027.6810658
ACGCAGA37100.026.9734527
GTGGTAT7500.026.63711
GCAGAGT37550.026.560179
CAGAGTA38300.025.77524610
GTACTAG801.0857612E-425.395541
AGTACGG34950.024.28846413
GAGTACG36200.023.54319812
TACTAGG1152.0200805E-623.5248532
TGGTATC8350.023.4896372
GTAATAC6000.023.1381591
TTAACGG902.3096411E-422.72076635
Produced by FastQC (version 0.11.5)