FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874633
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57140.3048063274251547No Hit
CATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA51080.27248000008535No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47770.2548232107297802No Hit
GCATAGATGAGGCGCCTAGGGGCTTCCCCTGGTTTCTGCTGATACCAGAC40590.21652238064730536No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40280.21486872363817344No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC37490.19998581055598613No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC37120.1980120908999255No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36020.19214427570623155No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT33540.17891501963317621No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33030.17619448713428174No Hit
CTGTAAGTCTGCTGACAATAGTAAGTCGCGAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC31970.17054004703854037No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31940.17038001571507597No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31530.16819292096106278No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31090.16584579488358522No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG31040.16557907601114458No Hit
GACTTACAGTTTCCCGCTCGCTTTCGGCGGAGGGACCATGGTGGAGATCAAAGGAACTGTGGCTGCACCAT30990.16531235713870396No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT29530.1575241660634375No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29480.15725744719099685No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT29020.15480363356454302No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCATGGTCCCTCCGCCGAAAGCG28640.15277657013399423No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28010.14941591234124224No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT26730.1425879092067621No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26480.1412543148445589No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCATGGTCCCTCCGCCGAAAGCGAGCGGGAAA25970.13853378234566446No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25810.13768028195385443No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25790.13757359440487818No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25780.13752025063039006No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25700.13709350043448504No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC24370.12999877842756424No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTCGCGACTTACTATTGTCAGCAGACTTACAGTTTC23540.12557124514504972No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23520.12546455759607347No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22990.12263733754820276No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22780.12151711828395212No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22590.12050358656867771No Hit
GTAGGTGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATGTTAGCAGATGGGTAGTCTGGTATCAGCAGAAA22150.11815646049120015No Hit
CCCCTAGGCGCCTCATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCA22130.1180497729422239No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21430.11431570872805503No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21140.11276873926789938No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT21050.11228864529750622No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21020.11212861397404185No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20640.11010155054349306No Hit
GTCTCCATCTTTCGTGTCTGCATCTGTAGGTGACAGAGTCACCATCACTT19670.10492720441814478No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19520.10412704780082288No Hit
GCCTTAGAGTCGAGGACACGGCCGTATATTATTGTGCCAGCCTCTATGAC19000.1013531715274403No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18960.1011397964294878No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTCGCGACTTACTATTGTCAGCAGACTTACAGTTTCCCGCTCGCTTT18920.1009264213315353No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18750.10001957716523716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4250.049.4261781
GTATCAA38400.039.262961
CGTATGC2200.039.130541
TCGTATG2250.038.1998240
CTAGCGC2250.036.1994528
ACTAGCG2300.035.40020427
TATCAAC43650.034.5369222
ATCAACG46400.032.489153
CCTTTGT13600.032.39215570
TCAACGC46650.032.312454
CAACGCA47500.031.7342285
AACGCAG47850.031.4288026
ACGAGAC2600.031.2771322
AATGGTA3100.030.6077770
CTTATAC3000.030.4927621
CGCAGAG50300.029.6959658
GACTAGC2800.029.06631726
AGAGTAC51850.028.80823511
CAGAGTA52600.028.3974710
TTATACA3150.027.962162
Produced by FastQC (version 0.11.5)