FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973521
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATTACTGTCAGCACTATGATACCTCACCTATGTACACTTTTGGCCA20140.20687792045574774No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19430.19958480608019755No Hit
GTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18970.19485968972420728No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCTGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18220.1871556956655275No Hit
CAGTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16460.1690769896078256No Hit
GTATTACTGTCAGCACTATGATACCTCACCTATGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA15880.1631192342024466No Hit
GTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAACGACTACTTACACTCTGA15470.1589077174503683No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15270.15685331903472038No Hit
CTATGATACCTCACCTATGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13800.141753490679708No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13430.1379528536107593No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12900.13250869780929225No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAAGTAGTCGTTACTTAGCCTGGTACC12010.12336662485965891No Hit
CCTCATCTACAGTGCATCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT11790.12110678660244617No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11780.12100406668166379No Hit
CATCTACAGTGCATCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11270.11576535072176151No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11240.11545719095941434No Hit
GTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC11230.11535447103863193No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGAT11220.11525175111784954No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11040.1134027925437664No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10880.11175927381124803No Hit
GTATCATAGTGCTGACAGTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC10710.11001303515794729No Hit
CTGTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAACGACTACTTACACTCTGA10330.1061096781682162No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT10210.10487703911882744No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT9900.10169272157457311No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC9870.10138456181222594No Hit
GAGTGTAAGTAGTCGTTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG9860.10128184189144354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)