FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493534
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24820.16618302629869827No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23020.15413107435116977No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTACTCATTTGCAGAAACA20980.14047219547730416No Hit
GTTAGTAGCCGCTTGGCCTGGTATCAGCAGAGACCAGGCAAAGCCCCTAA19720.13203582911403422No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18680.12507247909990668No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17990.12045256418668743No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17550.11750653148840268No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17110.11456049879011794No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16940.11342225888396247No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16920.1132883483067677No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16860.11288661657518342No Hit
TTATTATACTGTTGGCAGTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC16100.1077980146417825No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15550.10411547376892659No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15230.10197290453381042No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15150.10143726222503137No Hit
GTATAATAATTTCCCGTGGACCTTCGGCCAAGGGACCAAGGTGGAGATCCAACGAACTGTGGCTGCACCAT15110.10116944107064185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4750.039.8701441
GTATCAA27050.037.1701471
TATCAAC30650.032.5392532
ATCAACG30400.032.453333
TCAACGC30450.032.2849924
AACGCAG30900.031.9271326
CAACGCA30950.031.6544845
CGCAGAG34400.028.9785088
AGAGTAC34150.028.5924511
CAGAGTA34650.028.37260810
AACGGTT500.006891226428.26135365
GCAGAGT36900.026.8288829
AGTACGG32200.025.94622213
GAGTACG33550.024.90218512
CGGAATT9650.023.62043260
GTGTAAG1902.7284841E-1123.559631
GGTATCA25400.023.3170411
TACGGGC6350.023.28424315
CGGCTCT10700.022.50457421
GTACGGG36850.022.48553714
Produced by FastQC (version 0.11.5)