FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232656
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGA55530.4504906478368661No Hit
GTGTACTACTGTGCGAGAGGCCGCGTCCGATGGCCCCCCTTTGACTACTGGGGCCAGGGAAGCCTGGTCACCGT33650.27298775976428136No Hit
GTCTACAGTGGGTCCTTCAGAGGTTACTACTGCAACTGGATCCGCCAGCC30750.24946132578756766No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26850.21782232837060786No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25320.20541010630703133No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24190.19624290961955324No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23780.19291675860905233No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC21530.17466349086849858No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20810.1688224451915214No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19490.15811386145039655No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19390.15730260510637195No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18960.15381420282706612No Hit
GTATAACAGTGCCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18600.1508936799885775No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG18480.14992017237574798No Hit
CAGTAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGA18150.14724302644046677No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17370.1409152269570748No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTC17150.13913046300022067No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16630.1349119300112927No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16080.13045002011915732No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15890.1289086330655106No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15650.1269616178398515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15080.12233745667891123No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA14720.11941693384042262No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG14570.11820004932438571No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14460.11730766734595864No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14420.1169831648083488No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14400.11682091353954387No Hit
AGTCAGGACATTAGCCATTATTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATT14310.11609078282992173No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13970.11333251126023805No Hit
GAATAGATCAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGC13100.10627458106722396No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13020.10562557599200426No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12900.10465206837917472No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12830.10408418893835751No Hit
GTCCCTCACCTGCGAAGTCTACAGTGGGTCCTTCAGAGGTTACTACTGCA12710.10311068132552798No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT12700.1030295556911255No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG12570.1019749224438935No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12480.10124479173427137No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12420.10075803792785659No Hit
GGTTACTACTGCAACTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG12410.10067691229345413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCT150.00242906268.467574
TATGGAT18850.045.8973770
GTATCAA20000.042.797441
GTGGTAT2500.042.455061
TATACAC1750.041.0805443
AACGAGT350.00139849239.1338520
TCGTATG1600.038.82165540
TATCAAC23200.036.892852
CTTATAC1800.036.140061
TCAACGC24000.035.6601944
ATCAACG24050.035.5860563
CAACGCA24550.034.8612865
CGTATGC1800.034.5363841
AACGCAG24950.034.4395986
TATCTCG1700.034.41105736
TTATACA1900.034.2365652
CTCGTAT1800.032.56439239
CGCAGAG26850.032.0025338
AGAGTAC27050.031.63935711
CAGAGTA27450.031.55245210
Produced by FastQC (version 0.11.5)