FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005761151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232656
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGAGA52880.4289923547202139No Hit
GTGTACTACTGTGCGAGAGGCCGCGTCCGATGGCCCCCCTTTGACTACTGGGGCCAGGGAAGCCTGGTCACCGT30970.2512460897444218No Hit
GTCTACAGTGGGTCCTTCAGAGGTTACTACTGCAACTGGATCCGCCAGCC29240.23721135499279603No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28010.22723290196129334No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26980.21887696161783984No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22230.18034228527667087No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21790.17677275736296258No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC20770.16849794265391155No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19730.1600608766760556No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18530.1503258005477603No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17800.14440362923638062No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17520.14213211147311172No Hit
GTATAACAGTGCCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT17240.13986059370984283No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17170.1392927142690256No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTC17090.1386437091938059No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17050.13831920665619604No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16000.12980101504393765No Hit
CAGTAGTACACAGCCGTGTCCGCGGCGGTCACAGAGGTCAAGTTCAGGGA15980.12963876377513273No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15650.1269616178398515No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15370.12469010007658259No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA15150.12290533611972844No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14850.12047156708765462No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14750.11966031074363001No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13990.11349476252904298No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13640.11065536532495684No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13490.10943848080891992No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13320.1080593450240781No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13080.10611232979841903No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13030.10570670162640672No Hit
AGTCAGGACATTAGCCATTATTTGGCCTGGTATCAGCAGAAACCAGGGAAAGTTCCTAAGCTCCTGATCTATT12860.10432756584156488No Hit
GTCCCTCACCTGCGAAGTCTACAGTGGGTCCTTCAGAGGTTACTACTGCA12670.10278617878791813No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG12400.10059578665905168No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG12390.10051466102464922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)