FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443169
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17470.12105304368372659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15550.037.845721
GTATCAA33850.037.719531
GTGGTAT5650.034.7198141
TATCAAC37850.033.792582
TCAACGC37950.033.508394
ATCAACG38750.032.9894183
CAACGCA38600.032.9464155
AACGCAG40000.031.8792176
ACGCAGA42500.030.084847
AGAGTAC43850.028.84709211
CGCAGAG44450.028.7650368
CAGAGTA45800.028.0672510
GCAGAGT47350.027.074949
TGGTATC7550.025.958062
AGTACGG39100.024.9678113
GAGTACG39800.024.61504612
TACGGGG19250.022.3227815
GTACGGG45200.021.52221114
TAACCCG1000.003544759720.82811769
AAATCGC1451.126839E-519.2916756
Produced by FastQC (version 0.11.5)