FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666920
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTA551.4718717E-743.89638533
GTATCAA18750.040.2862051
GGTATCA7500.037.9972151
CTCGTAT1102.7284841E-1134.62303539
TATCAAC21850.034.5653572
ATCAACG21950.034.097643
TCAACGC21950.034.097644
CAACGCA22950.032.462315
AACGCAG23700.031.5775156
TGGTATC2750.031.2088032
GTGGTAT2950.030.2612081
CGCAGAG25750.029.0635768
AGAGTAC26050.028.72671711
TCGTATG1452.1827873E-1128.67522840
ACGCAGA26350.028.4017877
CGTATGC1503.274181E-1127.7382841
GTATTAG2000.027.4678691
CAGAGTA27500.027.33686610
CTTATAC1900.027.1064471
GCAGAGT27750.027.0926159
Produced by FastQC (version 0.11.5)