FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357984
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8570.2393961741306874No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6990.1952601233574685No Hit
GTAATAAAGACCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGA6230.1740301242513632No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5990.16732591400732993No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5930.16564986144632163No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5920.16537051935282024No Hit
AGCCAGGACAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG5850.16341512469831054No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5770.16118038795029946No Hit
GTCTCTGGTGCCTCCATCAGCACTTATACTGCCTACTGGGGCTGGATCCG5400.15084473049074817No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTGTCAGTGTAAAATCTGTGCCTGATCCACTGGCA5380.15028604630374542No Hit
GTGTAAAATCTGTGCCTGATCCACTGGCACTGAACCTGTCAGGGACCCCG5250.14665459908822742No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5180.14469920443371773No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4960.13855367837668722No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG4910.1371569679091803No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA4880.13631894162867614No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4870.13603959953517475No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG4860.13576025744167336No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT4860.13576025744167336No Hit
GTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGT4810.13436354697416644No Hit
GATTTTACACTGACAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT4810.13436354697416644No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA4790.13380486278716366No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4730.13212881022615536No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGCACTTATACTGCCTACTGGG4720.13184946813265397No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4710.13157012603915258No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4660.13017341557164566No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC4600.12849736301063736No Hit
CTACAAACTCCCCTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT4470.12486591579511934No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4410.12318986323411102No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG4400.12291052114060963No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA4350.12151381067310271No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA4310.12039644229909717No Hit
GGCCAAAAGTGTACAGGGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTA4290.11983775811209439No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG4270.11927907392509163No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC4210.11760302136408331No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG4190.11704433717708053No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4170.11648565299007776No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4110.11480960042906944No Hit
GTCCCTGACAGGTTCAGTGCCAGTGGATCAGGCACAGATTTTACACTGAC4040.11285420577455975No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT4030.11257486368105836No Hit
GTAGTAGGTGGTCCCAGCATAAAAGATGCTCCCAATGTATTCCAGCCCCTTCCCTGGGGACTGGCGGATCCAGCC4010.11201617949405561No Hit
GTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA4000.1117368374005542No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT3870.1081053901850362No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3840.10726736390453205No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3800.10614999553052651No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA3760.10503262715652097No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG3700.10335657459551265No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA3690.10307723250201127No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGTCCTGGCTTCTGCAGGTA3680.10279789040850988No Hit
ATGTAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA3670.10251854831500849No Hit
GGTCTAGTCAGAGCCTCCTACATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTC3620.10112183784750156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG150.00216375470.4832362
TACGCGC150.002397803868.681524
TTCGAGT200.006939603552.51612556
GGATACC200.007482411351.5255321
TGGTATC751.8189894E-1145.794082
GGTATCA3050.045.0433541
TATTCTA609.2716236E-442.52646670
CGCAATC350.001212836840.2761362
AAGTTAC350.001318960539.59502441
CAATAAC350.001374713439.2630420
GTGTATG350.001376603439.2520641
TGTGTAT350.001377549339.24658212
GTATCAA6600.035.906721
AATGGTA750.002774776234.0211770
GTGGTAT959.151336E-932.53791
GCGCAAT450.00418530631.28999561
TATACTC553.342023E-431.2188745
CAAGTTA450.00453671930.77877840
ACGGAAC450.0046249430.65787534
CCAACGG450.0046440230.63208831
Produced by FastQC (version 0.11.5)