FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357984
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8590.23995485831769017No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6440.17989630821489228No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5930.16564986144632163No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTGTCAGTGTAAAATCTGTGCCTGATCCACTGGCA5740.1603423616697953No Hit
GTAATAAAGACCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGA5710.15950433538929115No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5600.1564315723607759No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5540.1547555197997676No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5480.15307946723875926No Hit
AGCCAGGACAGTCTCCACAGCTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG5480.15307946723875926No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5280.14749262536873156No Hit
GTGTAAAATCTGTGCCTGATCCACTGGCACTGAACCTGTCAGGGACCCCG5280.14749262536873156No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5220.14581657280772325No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5070.14162644140520247No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC4870.13603959953517475No Hit
GTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTACTATGT4650.12989407347814427No Hit
GATTTTACACTGACAATCAGTAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACT4610.12877670510413872No Hit
GTCTCTGGTGCCTCCATCAGCACTTATACTGCCTACTGGGGCTGGATCCG4530.12654196835612763No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4510.12598328416912488No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA4490.1254245999821221No Hit
GGTCTAGTCAGAGCCTCCTACATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTC4420.12346920532761241No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC4410.12318986323411102No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA4380.12235183695360687No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4290.11983775811209439No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGCACTTATACTGCCTACTGGG4290.11983775811209439No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG4260.11899973183159024No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA4140.11564762670957363No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4140.11564762670957363No Hit
CTACAAACTCCCCTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT4120.11508894252257083No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4110.11480960042906944No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4060.11341288996156254No Hit
GGCCAAAAGTGTACAGGGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTA4060.11341288996156254No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG4000.1117368374005542No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG3990.11145749530705282No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3920.10950210065254314No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3910.10922275855904173No Hit
GTAGTAGGTGGTCCCAGCATAAAAGATGCTCCCAATGTATTCCAGCCCCTTCCCTGGGGACTGGCGGATCCAGCC3880.1083847322785376No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA3810.1064293376240279No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT3790.10587065343702512No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3780.10559131134352373No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG3780.10559131134352373No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3760.10503262715652097No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA3760.10503262715652097No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA3750.10475328506301958No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3730.10419460087601681No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT3720.10391525878251542No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG3660.1022392062215071No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC3630.10140117994100295No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT3610.10084249575400019No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGACCAAGAACCAATTCTCCCTGAAG3580.10000446947349602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGC200.00742260351.63018830
TGCGACA200.007526053651.449792
GTGGTAT750.050.3486821
TGGTATC800.047.1623082
CACCGAA350.00107179841.30096467
GTATCAA6650.040.78141
TTAATGC350.001223322740.20568561
TATCAAC7250.036.901922
GGTATCA2750.036.201121
AACGCAG7550.035.8849076
ATCAACG7650.035.4059223
TAATGCA400.002337247835.24059762
CAACGCA7700.035.185855
TCAACGC7700.035.180934
GTACCTA400.00265806334.3286481
AGAGTAC8200.032.6358211
CAGAGTA8250.032.4244310
ACGGGGG851.259832E-732.30479416
CGCAGAG8350.032.036118
GCAGAGT8450.031.6569849
Produced by FastQC (version 0.11.5)