FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098759
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65810.3135662551059936No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACCTCAGGGAGA63950.3047038750042287No Hit
GTCTCTGGTGGCTCCATCACTAGTTACTCCTGGAACTGGATCCGGCAGCC55550.26468022293174204No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG48420.2307077658749766No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48040.22889717209074503No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45900.2187006702532306No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT45580.21717595969808823No Hit
GTGTATTACTGTGCGAGACTTGTCAGGACCCAAGGTCCAAGGGGGACCTTCTACCCGATTATTGACTCCTGGGGC45120.2149841882750711No Hit
GAGTTACAGTACCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT44440.21174417834539366No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCAAGTCGGAGCATTGGCACCTATTTGAATTGGTGTCAGCAGAAA43510.2073129882945112No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43500.207265341089663No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA42230.20121414607394178No Hit
GTACTGTAACTCTGCTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT40160.19135117467036472No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG37960.1808687896037611No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37910.1806305535795201No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT37900.1805829063746719No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC37640.1793440790486187No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37520.17877231259044035No Hit
CTGTAACTCTGCTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC37500.17867701818074397No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC37250.177485838059539No Hit
GCATAGATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGACACCAATT37050.17653289396257502No Hit
GTCTCCATCCTCCCTATCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCAAGTCGGAGCATTGGCAC36580.1742934753347097No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC36280.17286405918926376No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35900.1710534654050322No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35430.16881404677716688No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT35160.16752757224626555No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC34160.16276285176144567No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33870.16138108282084795No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCACTAGTTACTCCTGGAACTGGATCCGGCAGCCCCCAGGGAAGGGA33820.16114284679660695No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31930.15213752508029746No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAACCTCAGGGAGAACTGGTTCTTGGACGTGTCTG31920.15208987787544925No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30700.14627691888396904No Hit
GTTACAGTACCCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAA30480.14522868037730868No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29530.14070219591672983No Hit
GATCAGGAGCTGAGGGGCTTTCCCTGGTTTCTGCTGACACCAATTCAAAT29330.13974925181976589No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA28480.135699239407669No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGGGTA28470.1356515922028208No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT27400.13055334128406357No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC27110.12917157234346582No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26970.12850451147559105No Hit
GTATTACTGTGCGAGACTTGTCAGGACCCAAGGTCCAAGGGGGACCTTCT26570.12659862328166313No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25980.12378743819561941No Hit
GTCCAAGGGGGACCTTCTACCCGATTATTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCTTCCA25930.12354920217137841No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25800.12292978850835184No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25530.12164331397745047No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25470.1213574307483613No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25440.1212144891338167No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25390.1209762531095757No Hit
GACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA24680.11759330156535364No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24660.11749800715565722No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAGCAGAGTTACAGTACC24180.11521094132294371No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23900.11387681958719416No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23520.1120662258029626No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACCCCA23500.11197093139326622No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23320.11111328170599864No Hit
CCTTGGTCCCTTGGCCGAACGTCGGAGGGGTACTGTAACTCTGCTGACAG23280.11092269288660586No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT23200.11054151524782026No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC23150.11030327922357927No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23130.11020798481388286No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTATCTGCATCTGTAGGAGACAGAGTCACCATCA23040.10977915997024909No Hit
GGGTTCCCTGGCCCCAGGAGTCAATAATCGGGTAGAAGGTCCCCCTTGGA22640.10787327177632115No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA22190.10572914755815221No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22190.10572914755815221No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT22170.10563385314845582No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTC21790.10382325936422428No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21720.10348972893028689No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21420.10206031278484096No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG21260.10129795750726978No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20990.1000114829763684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTC200.00738925551.69787633
GGTATCA14400.047.3118061
GTGGTAT4200.040.9626051
GTATCAA35350.040.5894781
TGGTATC4250.039.668242
TATCAAC40800.035.0807572
ATCAACG42050.034.3652153
TGGATAG26050.034.1678770
TCAACGC42600.033.9215354
CAACGCA43000.033.6075865
AACGCAG43400.033.2962536
AGAGTAC46250.031.02130711
CGCAGAG46550.030.969218
TAACGGC1251.2551027E-1030.34535236
ACGCAGA50650.028.4623267
CGTATGC1103.603236E-828.27780341
CAACGTT600.007021695428.15327369
AGTACGG48600.027.60788313
TTTGCGC500.00780804227.54787328
GCACTAG500.00783715827.526871
Produced by FastQC (version 0.11.5)