FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221613
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT7770.3506112006064626No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC3280.1480057577849675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.1308587492610993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.12995627512826413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.12905380099542896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.10513823647529702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTA250.001623429775.7447870
TTCGGAA252.0598245E-457.69258549
TACGAAA200.006484160753.41743544
CGGGCTA200.00864603649.6690526
AGGGGGG2500.045.44686570
CTTATAC1550.044.882631
TCTCGTA1600.043.38797838
TCGTATG1700.043.22513240
GCCGTCT1800.041.80047646
TATACAC1750.041.6461943
TTATACA1700.040.9223982
CGCGTAG1750.040.0788828
ACGCGTA1750.039.97879827
GACGCGT1750.039.90632626
GCGTAGT1800.039.08120329
AAGGGGG2000.038.83562569
CTCGTAT1800.038.70440739
CCCACGA1800.038.64020519
GTCGCTC350.001577912438.1703628
CGCTATG350.001642477137.8601722
Produced by FastQC (version 0.11.5)