FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847194
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19940.23536521741183247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25700.039.784581
GGTATCA11000.037.1191441
TATCAAC30500.033.489732
TCAACGC30800.032.9193534
CAACGCA31300.032.7163435
ATCAACG31250.032.5550233
AACGCAG32100.031.6929786
AGAGTAC33150.030.89419211
ACGCAGA33100.030.7354857
CGCAGAG33800.030.1968358
CAGAGTA34750.029.66211510
GCAGAGT35200.029.1872169
AGTACGG32300.027.01369513
GAGTACG33600.026.16905212
GTGGTAT5350.024.5989251
TACGGGT5750.024.02306415
GTACGGG36050.024.01677114
AATAGCG1852.237357E-1023.80126862
TACGGGG15450.023.76897215
ACGGGTC2053.6379788E-1223.01248616
Produced by FastQC (version 0.11.5)