FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902353
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19040.21100389758775115No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9100.10084745105296929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCG450.001640184537.88248469
GTATCAA24350.035.9141121
ATCAACG25300.034.6428763
TCAACGC25600.034.2312134
CAACGCA25700.033.9596945
CGATTAA1201.7797622E-533.1018270
GGTATCA10900.032.90691
AACGCAG26700.032.815486
AGAGTAC26900.032.323411
TATCAAC27150.032.294842
GTGGTAT3150.031.4493451
CGCAGAG28450.030.7969538
CAGAGTA28600.030.3970210
ACGCAGA28850.030.3699597
GCAGAGT29350.029.7364239
AGTACGG26700.027.96803713
TACGGGG12700.027.65582715
CCGTTAA1005.548536E-727.28294816
GAGTACG27750.026.91127612
GTACGGG29000.025.7498814
Produced by FastQC (version 0.11.5)