FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546032
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT10080.1846045653002022No Hit
GTGTATTACTGTCAGCACTATGATACCTCACCTATGTACACTTTTGGCCA8910.16317724968500016No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8770.160613297389164No Hit
GTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8550.1565842294957072No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCTGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA7990.14632842031236265No Hit
GTATTACTGTCAGCACTATGATACCTCACCTATGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA7830.14339818911712135No Hit
CTATGATACCTCACCTATGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT6890.12618308084507868No Hit
GTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAACGACTACTTACACTCTGA6540.11977320010548831No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6480.11867436340727285No Hit
CAGTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6400.11720924780965218No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6320.11574413221203154No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6270.11482843496351863No Hit
GTATCATAGTGCTGACAGTAATACACTGCACAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC5480.10036041843701467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG250.001057509484.40999670
TTATACA2250.050.4794542
CGTATGC2100.049.223541
CTCGTAT1950.049.1693439
TCGTATG2200.048.4059340
TATACAC2350.048.3313943
ATGCCGT2250.044.79564344
CTTATAC2700.042.0662161
ACGAGAC2650.041.61839322
TACACAT2950.040.762335
GCCGTCT2450.039.96017546
ATCTCCG2850.039.85220310
ACACATC2950.039.633676
TCTCCGA2900.039.16150711
TGCCGTC2500.039.02255245
CCGTCTT2650.038.4258447
GTATGCC2850.037.5819142
TAAGACA3000.037.00707231
ATCTCGT2650.035.99197837
AAGGGGG3450.035.6569969
Produced by FastQC (version 0.11.5)