FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219891
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.040.0397071
GTATCAA33800.040.0194441
TCAACGC39250.034.805884
TCGTATG2200.034.6306640
GACGGAC2200.034.26977526
CAACGCA39900.034.153065
ATCAACG39950.034.1103133
TATCAAC40850.033.2777142
AACGCAG42050.032.40556
ACGCAGA43400.031.3974937
CGCAGAG44300.030.7596238
CGTATGC2500.030.51039941
AGAGTAC44600.030.39918511
CAGAGTA46900.028.90839410
GCAGAGT47400.028.7479179
TCTATCG605.4560736E-428.71957235
TATGCCG2700.028.3091243
CTCGTAT2700.028.18952639
TCTCGTA2750.027.64721538
AGTACGG35400.027.56406213
Produced by FastQC (version 0.11.5)