FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219891
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20040.16427697228686824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34200.040.960851
GGTATCA13300.039.6589161
TCAACGC38650.035.4877744
CAACGCA38850.035.482625
TATCAAC39550.035.2121122
ATCAACG39500.034.637523
AACGCAG41050.033.8309826
ACGCAGA43250.032.1891867
CGCAGAG44100.031.1796578
AGAGTAC44600.030.98603211
CAGAGTA46250.029.80418210
GCAGAGT46100.029.7527649
GTGGTAT5000.029.4557571
AGTACGG38550.027.33038913
GAGTACG38950.027.31318912
TGGTATC4900.025.8374582
GTACGGG42550.024.51996214
TACGGGA13500.023.56603215
CGAATTA1803.8198777E-1022.8058415
TACGGGG17200.022.0765815
Produced by FastQC (version 0.11.5)