FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198991
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT5910.29699835670959995No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT2510.12613635792573533No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA2300.115583116824379No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC1990.10000452281761488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC850.055.5002021
TTATACA950.053.1917082
GTACGAA200.007309491351.8221443
TAGACAC200.00807574550.532125
TATACAC1050.048.113733
ATACACA1950.041.451834
CAACCGA350.001313990339.61633744
GTGGTAT603.4820368E-739.3126451
GGTATCA2650.038.1470451
TACACAT1250.037.730655
GTAATAC901.5643309E-1037.4406131
GTATCAA4850.035.433491
AATACAC1151.8189894E-1235.1439443
TGTTAGA951.6959825E-435.01215470
ATCTCCG1350.034.93578710
CACGAGA1350.034.92700621
GCCGTCT1102.3646862E-1134.8808946
TCGTATG1102.910383E-1134.38847440
CCCTAAG1400.033.7985628
GACCCTA1400.033.76449226
Produced by FastQC (version 0.11.5)