FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710051
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA7920.11154128365427274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16350.040.837961
GGTATCA6700.039.040811
TATCAAC18150.036.595712
ATCAACG19250.034.6833043
TCGCGCG400.00254654134.63466641
TCGTATG601.3122075E-534.61258740
TCAACGC19300.034.5934524
CAACGCA19600.033.8931475
AACGCAG19850.033.4615676
AGAGTAC21650.030.67954311
ACGCAGA21850.030.3987247
CGCAGAG22000.030.347898
AGTACGG20000.029.24874513
CAGAGTA22850.029.06836310
GCAGAGT23150.028.8403289
TGTTCGC605.492018E-428.68533925
GAGTACG20900.028.15387312
CGACCGT658.664251E-426.53297832
ACCGATC951.0656113E-525.37281627
GTACGGG23050.025.22923714
Produced by FastQC (version 0.11.5)