FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710051
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8330.11731551677273885No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA7190.10126033200432083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC200.00737225651.72443835
GGTATCA5950.041.0519371
GTATCAA15650.039.5685771
TATCAAC17100.036.180172
ATCAACG18100.034.726473
CAACGCA18600.033.9728435
AACGCAG18850.033.704466
TCAACGC18750.033.701064
ACGGGTA652.3884239E-531.71157616
ACGCAGA20200.031.6219467
CGCTATT450.004155037531.34073863
AGAGTAC20500.031.16357611
CGCAGAG21200.030.1282228
CAGAGTA21600.029.57029210
GCAGAGT22100.028.9012819
TAACGAC500.007291966627.93401154
GAGTACG20450.027.88065312
AGTACGG20350.027.8508413
GTATTAT951.0732852E-525.3493441
GTACGGG22450.025.2456414
Produced by FastQC (version 0.11.5)