FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894120
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22300.036.1937641
GGTATCA10750.035.949811
TCGTATG1500.034.6182740
CGTATGC1550.033.53382541
CTCGTAT1450.033.37163539
TATCAAC25600.031.793572
TCAACGC26500.030.8428364
CAACGCA26950.030.2009415
ATCAACG27250.029.9939543
AACGCAG27950.029.1187826
TGGTATC4600.028.2506242
ACGCAGA30050.027.1976537
CGCAGAG30600.026.7073158
CTTATAC2200.026.4273031
AGACCGA1950.026.32539225
GTGGTAT5200.026.3077221
AGAGTAC31250.026.15180211
GCCGTCT2150.025.92831246
CAGAGTA32800.024.9159710
TCTCGTA1851.8189894E-1124.26590338
Produced by FastQC (version 0.11.5)