FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894120
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14930.16697982373730594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21250.040.889191
CACGCGA458.104887E-539.7344964
TATCAAC24050.035.946192
TCAACGC24850.034.4985124
CAACGCA25000.034.1549035
ATCAACG25550.033.41783
GGTATCA9050.032.8854941
AACGCAG26150.032.783486
ACGCAGA27500.030.9239837
CGCAGAG28250.029.7403038
AGAGTAC28800.029.29257211
CAGAGTA29700.028.2899210
AGTACGG23650.027.1506213
GCAGAGT31150.026.8619049
GAGTACG24200.026.39242612
TCGCGGT659.056604E-426.3333927
GTGGTAT4950.026.2610531
TGGTATC4600.025.9990582
GTACGGG26600.023.75567814
TACGGGG10900.023.1890315
Produced by FastQC (version 0.11.5)