FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349191
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36700.040.088921
GGTATCA15000.038.1819731
CAACGCA41400.035.2051055
GTGGTAT5850.035.174551
TCAACGC42400.034.535284
ATCAACG42550.034.332943
TATCAAC43250.034.1762432
AACGCAG43300.033.7357566
ACGCAGA44950.032.4974027
CGCAGAG46350.031.5158218
TCGTATG2150.030.59657740
AGAGTAC47650.030.58403411
TGGTATC6400.030.0082862
CAGAGTA48650.029.95538110
GCAGAGT50950.028.7377289
CTCGTAT2100.028.00044839
GAGTACG40850.027.19603512
AGTACGG40750.027.1786313
TATACAC3450.026.837723
CGTATGC2650.026.15363141
Produced by FastQC (version 0.11.5)