FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349191
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23590.1748455185366638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34400.040.1616631
TCAACGC38800.034.9550324
ATCAACG39350.034.6379663
CAACGCA38950.034.5553475
GGTATCA13850.033.9106331
TATCAAC40500.033.745232
AACGCAG40400.033.3151176
ACGCAGA41750.032.237867
CGCAGAG43550.031.0603898
AGAGTAC45450.029.68768511
CAGAGTA46600.029.17227610
GCAGAGT47800.028.3693319
ACGGGTA3050.026.95292916
GTGGTAT6300.025.5753971
AGTACGG42250.025.53371413
GAGTACG42400.025.280912
TACGGGT9300.024.67483115
TTAACGG3500.023.62949435
TGGTATC6700.023.0055312
TAACGGC3600.022.99284636
Produced by FastQC (version 0.11.5)