FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556580
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA7060.126846095799346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAA200.00640108153.6036164
CTCGTAT950.047.33167339
TCGTATG900.046.1936640
CGTATGC1050.039.64874341
GTATCAA21550.037.112341
GGTATCA9900.033.4877741
GCTAATC1253.6379788E-1232.98004533
TCAACGC24700.032.9269684
ATCAACG25050.032.4669043
CAACGCA25200.032.273655
TATCAAC25650.031.976772
AACGCAG25950.031.6014186
ACGCAGA26950.030.3020367
CGCAGAG27400.029.8043758
AGAGTAC27850.029.44548811
ACGAGAC1401.6370905E-1129.30090322
TATGATC605.0274044E-429.12038247
GCGACCG1202.752131E-928.67772735
TGCGACC1202.7830538E-928.64662434
CTACGTC500.0065108528.58859364
Produced by FastQC (version 0.11.5)