FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081561
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA31730.2933722647173853No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24820.22948312670297838No Hit
GGCCTACAGTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCA23930.22125427969388686No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC21690.20054347373842066No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA21650.20017363791778736No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19650.18168184688612107No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17810.1646693991369881No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT16480.15237235810093003No Hit
GTGTACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTC16220.14996842526681342No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16120.1490438357152301No Hit
GGTACACACTGGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14350.13267860065220546No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13780.12740844020818057No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13700.12666876856691395No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13390.12380254095700567No Hit
GGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13080.12093631334709738No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13040.12056647752646406No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13030.12047401857130573No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12860.11890221633361409No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12760.11797762678203078No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12730.1177002499165558No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12600.11649828349949748No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGATACACTGCCG12540.11594352976854749No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATGACAACATCC12480.1153887760375975No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC12020.11113566410031427No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11910.11011861559357262No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11730.10845435440072265No Hit
GTATCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGA11230.1038314066428061No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11010.1017973096293228No Hit
GTACACACTGGCCGTACACTGTAGGCCAGGGGACCAAGCTGGAGATCAAA11000.10170485067416447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.043.9810071
TCGTATG2150.041.6405440
CGTATGC2250.041.40823741
ACTCGAC2400.040.80166227
GTGGTAT2800.038.524721
TCGACGT2550.038.44964629
GTATCAA28300.038.235441
CGACGTC2450.037.30579830
ACGAGAC2900.033.7200422
TATCAAC32450.033.447882
CGAGACT3050.033.17185623
GACTCGA2950.033.17152826
TATACAC3100.032.6187173
ATCAACG34150.031.7813633
TCAACGC34050.031.775714
ATGCCGT2850.031.69144244
CAACGCA34350.031.4981925
ATACACA3750.031.4589424
TACACAT3350.031.1906365
AACGCAG35100.030.8237296
Produced by FastQC (version 0.11.5)