FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083954
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25800.041.3090861
GGTATCA10250.041.255791
TATCAAC30900.034.9329032
ATCAACG31000.034.595243
TCAACGC31250.034.203754
CAACGCA31750.033.7749145
AACGCAG32500.032.9954956
CGTATGC1550.031.28952241
CTCGTAT1700.030.51395239
ACGCAGA35100.030.4534637
AGAGTAC35950.029.82902711
GTGGTAT3700.029.73391
CGCAGAG36250.029.487358
CAGAGTA36750.029.27321410
TGGTATC3850.028.57282
TCGTATG1850.028.06068440
GCAGAGT39900.026.8760289
AGTACGG33700.025.90521413
GAGTACG34400.025.37807512
TAACGGC1401.3758836E-824.66285136
Produced by FastQC (version 0.11.5)