FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083954
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14210.13109412392038775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24800.042.5013731
ATCAACG27750.037.5582473
CAACGCA27850.037.3037265
TCAACGC27900.037.233444
GGTATCA9500.037.2245861
TATCAAC28900.036.077032
AACGCAG29400.035.570286
GTGGTAT3850.034.7792471
ACGCAGA31450.033.3607337
TGGTATC4100.032.623892
CGCAGAG32200.032.477218
AGAGTAC33000.032.10845611
CAGAGTA34350.030.64406810
CTCGACA900.00605094929.02090370
GCAGAGT36350.028.769369
AGTACGG32100.028.20420513
GAGTACG32950.027.68350812
CTATCCG500.00797154627.4299359
TACGGGT6400.026.79693615
GTACGGG36800.024.32246614
Produced by FastQC (version 0.11.5)