FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670624
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12790.19071789855418236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9400.042.8333741
GTATCAA23650.040.2532731
ATCAACG27400.034.7078823
TCAACGC27250.034.6513444
TATCAAC27600.034.5927732
CAACGCA28050.033.5390435
AACGCAG29850.031.4023956
CGCAGAG30250.031.0998388
ACGCAGA30650.030.6939687
TGGTATC3150.030.3098562
AGAGTAC30950.030.29309811
CAGAGTA31500.029.86571910
AGTACGG24600.029.52218613
GAGTACG24650.029.46230312
TACGGGA9600.029.12370715
GCAGAGT32500.028.9467749
GTGGTAT3450.027.6928141
GTACGGG26800.026.58982714
CGGGATT1351.0642907E-825.26198417
TACGGGG9550.024.27781315
Produced by FastQC (version 0.11.5)