FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452560
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCCCGGCGCTCAGGCTGTTCATTTGAAGATACA13660.30183843026339047No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11500.25410995227152205No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCA10190.22516351423015735No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8210.18141240940427789No Hit
GTATATTACTGTGCAAGAGAAGGGTTCGACTACGGTGACCACGCGGCTCA8150.1800866183489482No Hit
GTCTTTGTCTCCAGGGGAAGTAGCCACCCTCTCCTGCAGAGCCAGTCAGACTGTTAGCGGCTTCTTAGCCTGGTA7920.17500441930351776No Hit
GTCTGGGACAGGCTTCACTCTCACCATCAGCGGCCTAGAGCCTGAGGATT7390.16329326498143892No Hit
CTCCTGCAGAGCCAGTCAGACTGTTAGCGGCTTCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT7330.16196747392610925No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7330.16196747392610925No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7300.1613045783984444No Hit
GTCCCAGACCCACTGCCACTGAACCTGACTGGGACGCCAGTGGCCCTGTT7030.15533851864946083No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6660.14716280714159446No Hit
GGCTTCACTCTCACCATCAGCGGCCTAGAGCCTGAGGATTTTGCAGTTTATTTCTGTCAGCACCGTGGCGACTGG6560.14495315538271167No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6490.14340639915149372No Hit
GTCTCCAGCCACACTGTCTTTGTCTCCAGGGGAAGTAGCCACCCTCTCCTGCAGAGCCAGTCAGACTGTTAGCGG6000.132579105532968No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5800.1281598020152024No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5700.12595015025631962No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5690.12572918508043132No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5650.12484532437687822No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC5410.11954216015555948No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5250.11600671734134702No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5080.11225030935124625No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG5070.11202934417535795No Hit
GCCTAGAGCCTGAGGATTTTGCAGTTTATTTCTGTCAGCACCGTGGCGAC5030.11114548347180483No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5010.11070355312002829No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4930.10893583171292204No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4900.1082729361852572No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCC4850.1071681103058158No Hit
GTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGAACCGAGGCCAGTCGCCACGGTG4800.10606328442637442No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4790.10584231925048612No Hit
GAGTGAAGCCTGTCCCAGACCCACTGCCACTGAACCTGACTGGGACGCCA4770.10540038889870958No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4640.10252784161216193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3800.044.2843781
GTATCAA10150.043.3093871
TATCAAC11700.037.8486062
AATATAC3650.035.7345923
ATCAACG12600.035.141253
ATATACA3750.034.7778244
TCAACGC12850.034.4537624
CGTACTA905.3823896E-934.36198829
TGGTATC1102.910383E-1134.3278052
AACGCAG13000.034.0524566
TATACAG3750.033.8626175
GTAATAT3900.033.4624021
CAACGCA13250.033.413655
CCGTGTC3950.033.01327512
GTGGTAT1253.6379788E-1232.969271
AAGATAC4000.032.9390643
ACTAGAT959.0039975E-932.59673332
GTACTAG959.082214E-932.5678730
GCCGTGT3900.032.55661411
ACCGTAC959.131327E-932.54986227
Produced by FastQC (version 0.11.5)