FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553877
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15500.042.691761
TATCAAC18050.037.413562
CAACGCA18450.036.410055
ATCAACG18900.035.912313
TCAACGC18750.035.8274884
AACGCAG19150.035.437086
GGTATCA6200.034.839131
TCGTATG501.8058205E-434.67445840
ACGCAGA20450.032.8491637
CGCAGAG21300.031.696338
CTCGTAT553.1854314E-431.47910539
AGAGTAC21450.031.47467411
CAGAGTA21900.030.82793810
CTTATAC1501.8189894E-1229.7146431
GCAGAGT23000.029.5025629
CCGCGTG850.00631204228.76695870
GAGTACG16750.028.43952812
AGTACGG16900.028.18710513
ACGGGTA1005.3063195E-727.41899716
TACGGGG7200.027.13338315
Produced by FastQC (version 0.11.5)