FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005761200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005761200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553877
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6660.12024330311603479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACG306.548347E-445.6531223
GTATCAA16250.039.233961
TCAACGC17600.035.799434
CAACGCA17750.035.303985
TATCAAC18200.035.190032
AACGCAG18050.034.5275046
ATCAACG18350.034.519733
ACGCAGA19200.032.4565167
GGTATCA7050.031.1166921
AGAGTAC20300.031.03793111
CGCAGAG20200.030.849768
CAGAGTA20750.030.36207810
GAGTACG18850.029.24728212
AGTACGG18800.028.9607813
TAACGGC605.3999026E-428.76695336
GCAGAGT22300.027.9446289
TACGGGA7200.027.11388815
ACGGGGG2950.026.70270216
TTAACGG658.658323E-426.53478635
CGTCCAA659.0423244E-426.3383410
Produced by FastQC (version 0.11.5)